Application
Sodium orthovandate was used to prepare the stop solution in dephosphorylation assay of insulin receptor kinase.26 It was one of the reagents used in the development of Matrix ChIP that utilizes surface-immobilized antibodies.27
Packaging
10, 50, 250 g in poly bottle
Biochem/physiol Actions
Sodium orthovandate suppresses the activation of p53-mediated apoptosis triggered in response to radiation. It reduces the detrimental effects of hematopoietic syndrome, hematopoiesis and delayed genotoxic effects of induced by total body irradiation of mice.24 In combination with menadione, orthovandate prevents the migration of detached human glioma cells in response to anti-cancer drugs.25
Inhibits ATPase, alkaline phosphatase and tyrosine phosphatase. Decavanadate, which is formed at acidic pH, inhibits inositol 1,4,5-trisphosphate (IP3)-induced Ca2+ release from endocrine cells and blocks IP3 binding to its receptor in brain tissue.
Features and Benefits
This compound is a featured product for Kinase Phosphatase Biology research. Click here to discover more featured Kinase Phosphatase Biology products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.
This compound is featured on the Phosphoprotein Phosphatases (Tyrosine) page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.
Safety Information
Symbol
GHS07
Precautionary statements
P301 + P312 + P330
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
RIDADR
NONH for all modes of transport
RTECS
YW1120000
Frequently Asked Questions
How do I prepare solutions of Product S6508, Sodium Orthovanadate?
Solutions of Product S6508, Sodium Orthovanadate, prepared as 1 mM in water (or higher if desired) and adjusted down to pH 10 can be aliquotted in plastic tubes and stored at -20°C. To ensure the presence of monomers, solutions should be heated to boiling until translucent and pH readjusted to 10. View the product information sheet (under Documents, above), which has more details.
How do I store solutions of Product S6508, Sodium Orthovanadate?
Solutions of Product S6508, Sodium Orthovanadate, prepared as 1 mM in water (or higher if desired) and adjusted down to pH 10 can be aliquotted in plastic tubes and stored at -20°C. To ensure the presence of monomers, solutions should be heated to boiling until translucent and pH readjusted to 10. View the product information sheet (under Documents, above), which has more details.
What is the amount of S6508, Sodium orthovanadate, needed for inhibition?
For ATPase, a 0.5-1 μM concentration of sodium orthovanadate inhibited 50% of the activity.Please see the product information sheet (under Documents, above) whichprovides referenceson several other enzymes.
Which enzymes does S6508 inhibit?
Sodium orthovanadate is an inhibitor of ATPase, alkaline phosphatase and tyrosine phosphatase.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.