General description
The trypan blue dye is an azo dye,[5] derived from toluidine.[4] It is soluble in water and has a molecular weight of 961 g/mol.[5]
Application
Trypan blue has been used:
• in cell viability assay[6]
• to determine the number of living and necrotic cells by staining with 0.2% trypan blue solution.[2][3]
• to stain plant tissue in order to to visualize cell death[3]
Packaging
5, 25, 100 g in glass bottle
Biochem/physiol Actions
The trypan blue dye is useful to differentiate the dead and live cells based on the cell′s ability to take up or exclude the dye. A viable cell is assumed to possess an intact cell membrane and hence excludes this dye and the cytoplasm appears clear. While on the other hand non-viable cells shows a blue cytoplasm.[1]